PurposeMammalian retroviral expression of Myc-ER
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73570||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepMSCV IRES mCherry
Backbone manufacturerAddgene Plasmid #52114
- Backbone size w/o insert (bp) 6573
- Total vector size (bp) 7923
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1350
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
- Promoter MSCV
/ Fusion Proteins
- Mouse ER (C terminal on insert)
- IRES-mCherry (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pLXSN
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-human-c-MYC-ER-mouse IRES-mcherry was a gift from Martine Roussel (Addgene plasmid # 73570 ; http://n2t.net/addgene:73570 ; RRID:Addgene_73570)
For your References section:The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Vo BT, Wolf E, Kawauchi D, Gebhardt A, Rehg JE, Finkelstein D, Walz S, Murphy BL, Youn YH, Han YG, Eilers M, Roussel MF. Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 10.1016/j.ccell.2015.12.003. 10.1016/j.ccell.2015.12.003 PubMed 26766587
Map uploaded by the depositor.