Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pJK372
(Plasmid #73597)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73597 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pESC-URA
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 6631
  • Total vector size (bp) 8894
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    delta-12 fatty acid acetylenase
  • Alt name
    vFAD2
  • Species
    Crepis alpina
  • GenBank ID
    ABC00769.1
  • Promoter GAL10
  • Tag / Fusion Protein
    • FLAG tag (C terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer GAL10 forward primer
  • 3′ sequencing primer GAL10 reverse primer
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    delta-15 fatty acid desaturase
  • Alt name
    FAD3
  • Alt name
    Fad3
  • Alt name
    omega-3 fatty acid desaturase
  • Species
    Crepis alpina
  • GenBank ID
    DQ176017 ABA55806
  • Promoter GAL1
  • Tag / Fusion Protein
    • c-myc tag (C terminal on backbone)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GAL1 forward primer
  • 3′ sequencing primer GAL1 reverse primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK372 was a gift from T. J. Kappock (Addgene plasmid # 73597 ; http://n2t.net/addgene:73597 ; RRID:Addgene_73597)