pET24a-AlkB deltaN11
(Plasmid #73622)

• Purpose: Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag
• Depositing Lab: Catherine Drennan
• Publication: Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):755-60. Epub 2007 Jan 9.

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5310
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AlkB
• Species: E. coli
• Mutation: deletion of first 11 AA
• Entrez Gene: alkB (a.k.a. b2212, ECK2204, aidD)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry