pME nls-eos
(Plasmid
#73791)
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PurposeMiddle entry clone with photoconvertible eos conjugated to a nuclear localization signal
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
- Total vector size (bp) 3272
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Vector typemiddle entry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenls-eos
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Eos is originally cited in the following paper: Wiedenmann J., Ivanchenko S., Oswald F., Schmitt F., Röcker C., Salih A., Spindler K.-D., Nienhaus G. U. (2004). EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. USA 101, 15905–15910.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME nls-eos was a gift from David Raible (Addgene plasmid # 73791 ; http://n2t.net/addgene:73791 ; RRID:Addgene_73791) -
For your References section:
Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest. Curran K, Lister JA, Kunkel GR, Prendergast A, Parichy DM, Raible DW. Dev Biol. 2010 Aug 1;344(1):107-18. doi: 10.1016/j.ydbio.2010.04.023. Epub 2010 May 9. 10.1016/j.ydbio.2010.04.023 PubMed 20460180
Map uploaded by the depositor.
