pMAL_dstβL
(Plasmid #73805)

• Purpose: Plasmid for selection for insertion and homodimerization
• Depositing Lab: Sarel Fleishman
• Publication: Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi: 10.7554/eLife.12125.

Backbone

• Vector backbone: pmal 27
• Backbone size w/o insert (bp): 6272
• Modifications to backbone: Removed MBP tag Changed MCS restriction sites Added Ampicillin resistance
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: CAT Marker
• Species: e.coli
• Insert Size (bp): 1006
• Promoter: NaN

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: GGAGTGGTGAATCCGTTAGCG

Gene/Insert 2

• Gene/Insert name: ToxR
• Alt name: ToxR
• Species: ecoli
• Insert Size (bp): 811
• Promoter: ToxR promotor
• Tag / Fusion Protein:
  • Part of the dsTbL chimera (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: GCCTGGGAATTCTTGAAGACGAAAGGG

Gene/Insert 3

• Gene/Insert name: beta lactamase
• Species: e. coli
• Promoter: ToxR promotor
• Tag / Fusion Protein:
  • part of the dstbl chiemra (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: CGACTCTGCGCAAAATGCTCAAAGATTCGACAAAGTCCCC

Gene/Insert 4

• Gene/Insert name: spectomycin resistance
• Species: e.coli
• Insert Size (bp): 966
• Promoter: NaN

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: GGTTTACACTTACTTTAGTTTTATGG

Gene/Insert 5

• Gene/Insert name: CLS TM domain
• Species: e.coli
• Insert Size (bp): 87
• Tag / Fusion Protein:
  • Part Of the dstbl chiemra (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The p-Mal plasmid was generously provided by the Mark Lemmon laboratory
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry