h-Plekhm1_EGFP
(Plasmid #73836)

• Purpose: Express human Plekhm1 in mammalian cells
• Depositing Lab: Paul Odgren
• Publication: Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7871
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Plekhm1
• Species: H. sapiens (human)
• Insert Size (bp): 3171
• Entrez Gene: PLEKHM1 (a.k.a. AP162, B2, OPTA3, OPTB6)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: CMV fwd primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry