Purposeexpression plasmid of AsCpf1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73917||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturergift from Silvia Aldaz
- Backbone size w/o insert (bp) 12429
- Total vector size (bp) 16571
Vector typeInsect Expression
Growth in Bacteria
SpeciesH. sapiens (human), D. melanogaster (fly)
Insert Size (bp)4059
- Promoter act5C
- Cloning method Gibson Cloning
- 5′ sequencing primer ATTTCTACATTACCAAATAAGG
- 3′ sequencing primer AAATCTCTGTAGGTAGTTTG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byhAsCpf1 from Feng Zhang (Addgene plasmid 69982).
Terms and Licenses
Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:act-AsCpf1 was a gift from Simon Bullock (Addgene plasmid # 73917 ; http://n2t.net/addgene:73917 ; RRID:Addgene_73917)
For your References section:Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Port F, Bullock SL. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. 10.1038/nmeth.3972 PubMed 27595403