pMSCV-BirA-P2A-mCherry
(Plasmid #74056)

• Purpose: retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein
• Depositing Lab: Ria Baumgrass
• Publication: Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.

Backbone

• Vector backbone: pMSCV-IRES-GFP
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 7443
• Modifications to backbone: added SV40 ori
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BirA biotin ligase
• Species: E.coli
• Insert Size (bp): 940
• Promoter: MSCV-LTRs
• Tag / Fusion Protein:
  • P2A-mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CGT TCG ACC CCG CCT CGA TCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pBY2982, containing the E.coli biotin ligase birA-mCherry fusion protein sequence was a gift from Ralf Baumeister (Addgene plasmid # 23220)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry