PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74130||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturergift from M. Bouvier (University of Montreal)
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameArr3, beta-Arrestin2
SpeciesR. norvegicus (rat)
MutationInsertion of FlAsH motif (CCPGCC) following amino acid residue 139 (see depositor comment below).
/ Fusion Protein
- Renilla luciferase (rLuc) (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Note: during Addgene's quality control, it was found that the FlAsH motif was inserted after amino acid 139, not 140. This difference has NO affect on plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Rlucrarr3-Flash2 was a gift from Louis Luttrell (Addgene plasmid # 74130 ; http://n2t.net/addgene:74130 ; RRID:Addgene_74130)
For your References section:The conformational signature of beta-arrestin2 predicts its trafficking and signalling functions. Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK, Laporte SA, Luttrell LM. Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 2016 Mar 23. 10.1038/nature17154 PubMed 27007854