pCDNA3 HA C9ORF72 op
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74156||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6833
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)1446
Entrez GeneC9orf72 (a.k.a. ALSFTD, DENND9, DENNL72, FTDALS, FTDALS1)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HIND3 (not destroyed)
- 3′ cloning site XHO1 (not destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH polyA (Common Sequencing Primers)
C9ORF72 sequence has been optimized, meaning it encodes same amino acid sequence than natural C9ORF72 (human) but with optimized nucleotide sequence to remove negative RNA element and rare codons.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3 HA C9ORF72 op was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74156 ; http://n2t.net/addgene:74156 ; RRID:Addgene_74156)