pHAGE-eGFP-Ago2
(Plasmid
#74231)
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PurposeGFP-Ago2 lentiviral vector
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74231 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHAGE
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAgo2
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SpeciesH. sapiens (human)
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Insert Size (bp)924
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Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGenerated by: Etienne Gagnon
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-eGFP-Ago2 was a gift from Carl Novina (Addgene plasmid # 74231 ; http://n2t.net/addgene:74231 ; RRID:Addgene_74231) -
For your References section:
Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins. Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD. RNA. 2012 Nov;18(11):2041-55. doi: 10.1261/rna.035675.112. Epub 2012 Sep 27. 10.1261/rna.035675.112 PubMed 23019594
Map uploaded by the depositor.
