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pGM34DEST
(Plasmid #74756)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74756 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC13T DNA
  • Total vector size (bp) 10758
  • Modifications to backbone
    unc-119(+) rescue cassette
  • Vector type
    Worm Expression
  • Selectable markers
    unc-119(+) rescue cassette

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gateway cassette
  • Insert Size (bp)
    1703

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kang Shen Laboratory, Stanford University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGM34DEST was a gift from Jordan Ward & Keith Yamamoto (Addgene plasmid # 74756 ; http://n2t.net/addgene:74756 ; RRID:Addgene_74756)
  • For your References section:

    A New Tool for Inducible Gene Expression in Caenorhabditis elegans. Monsalve GC, Yamamoto KR, Ward JD. Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705. Epub 2018 Nov 30. 10.1534/genetics.118.301705 PubMed 30504365