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Purposeexpress of GCN5 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEBB
- Backbone size w/o insert (bp) 5350
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGCN5
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Alt nameKAT2A
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SpeciesH. sapiens (human)
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Insert Size (bp)2514
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GenBank IDNM_021078
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Entrez GeneKAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
- Promoter EF-1alpha promoter
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CTC AAG CCT CAG ACA GTG G
- 3′ sequencing primer GAG GGG TGC AAG GAC CCA G (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBB Flag GCN5 was a gift from Ezra Burstein (Addgene plasmid # 74784 ; http://n2t.net/addgene:74784 ; RRID:Addgene_74784) -
For your References section:
GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA. Mao X, Gluck N, Li D, Maine GN, Li H, Zaidi IW, Repaka A, Mayo MW, Burstein E. Genes Dev. 2009 Apr 1;23(7):849-61. doi: 10.1101/gad.1748409. 10.1101/gad.1748409 PubMed 19339690
