pGAMA-YAP
(Plasmid #74942)

• Purpose: Lentiviral over-expression vector for YAP
• Depositing Lab: Miguel Ramalho-Santos
• Publication: Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3.

Backbone

• Vector backbone: pGAMA-empty
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YAP
• Alt name: YAP1
• Species: H. sapiens (human)
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: EF1α
• Tag / Fusion Protein:
  • T2A-mCherry

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

YAP introduced by Gateway reaction (with stop codon removed) will be driven by EF1a promoter, with a V5 C-terminal tag followed by T2A mCherry. Cells will turn red fluorescent.

Qin et al. YAP Induces Human Naive Pluripotency. 2016 Mar 15;14(10):2301-12.