pCIG1_UL136_25kDa_Myc
(Plasmid #74954)

• Purpose: Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.
• Depositing Lab: Felicia Goodrum
• Publication: Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 2014 Oct 8.

Backbone

• Vector backbone: pCIG1
• Backbone size w/o insert (bp): 7535
• Total vector size (bp): 7900
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UL136
• Alt name: HCMV UL136 protein, subcloned from TB40E
• Species: Human Cytomegalovirus strain TB40/E
• Insert Size (bp): 591
• Mutation: delta 1-77, M100A M128A
• GenBank ID: ABV71655.1 EF999921.1
• Entrez Gene: UL136 (a.k.a. HHV5wtgp125)
• Promoter: CMV, Lentiviral LTR
• Tag / Fusion Protein:
  • 3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-rev

Resource Information

• Supplemental Documents:
  • pCIG1_UL136_25kDa_Myc GenBank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by Dr. Katie Caviness. Expresses ONLY the 25kda isoform, expression of all other isoforms has been suppressed by mutating initiating methionine residues to alanine. Amino acids 1-77 are not present in this clone.