His-GINA3
(Plasmid
#74980)
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PurposeExpresses His tagged GINA3 protein in bacteria.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28b
- Backbone size w/o insert (bp) 5368
- Total vector size (bp) 6431
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3)
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Alt nameGINA3
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GenBank IDDQ120473.1
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Entrez GeneGnai3 (a.k.a. GIP3A)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His-GINA3 was a gift from Pradipta Ghosh (Addgene plasmid # 74980 ; http://n2t.net/addgene:74980 ; RRID:Addgene_74980) -
For your References section:
Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin. Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, Kufareva I, Abagyan R, Ghosh P. Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 10.1091/mbc.E14-05-0978. Epub 2014 Sep 3. 10.1091/mbc.E14-05-0978 PubMed 25187647
