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pJJR82
(Plasmid #75027)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75027 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDD282
  • Backbone size w/o insert (bp) 3854
  • Total vector size (bp) 10481
  • Modifications to backbone
    Replaced GFP with EGFP
  • Vector type
    Worm Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. The midiprep yield from this vector is generally low. The depositing lab uses the low-copy procedure of the plasmid isolation kit.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP^SEC^3xFlag
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    6627
  • Tags / Fusion Proteins
    • C. elegans codon optimized EGFP
    • 3xFLAG

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJR82 was a gift from Mike Boxem (Addgene plasmid # 75027)