pcDNA3-N-Flag-ASC1
(Plasmid #75134)

• Purpose: Expresses N-terminal Flag-tagged ASC1 in mammalian cells
• Depositing Lab: Bruce Beutler
• Publication: Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 Dec 7.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 5722
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PYD and CARD domain containing
• Alt name: Pycard
• Alt name: Asc
• Species: M. musculus (mouse)
• Insert Size (bp): 582
• Mutation: F23S (please see depositor comments below)
• GenBank ID: NM_023258
• Entrez Gene: Pycard (a.k.a. 9130417A21Rik, Asc, CARD5, TMS-1, TNS1, masc)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation F23S in ASC1 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.