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pcDNA3-optoB2-SS-YFP
(Plasmid #75270)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75270 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 7398
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arrestin independent version of optoB2
  • Mutation
    serine mutations at S362A,S363G
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-optoB2-SS-YFP was a gift from Michael Bruchas (Addgene plasmid # 75270 ; http://n2t.net/addgene:75270 ; RRID:Addgene_75270)
  • For your References section:

    Optodynamic simulation of beta-adrenergic receptor signalling. Siuda ER, McCall JG, Al-Hasani R, Shin G, Il Park S, Schmidt MJ, Anderson SL, Planer WJ, Rogers JA, Bruchas MR. Nat Commun. 2015 Sep 28;6:8480. doi: 10.1038/ncomms9480. 10.1038/ncomms9480 PubMed 26412387