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MAGE-A1Pro pGL4basic
(Plasmid #78136)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78136 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4basic
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAGE-A1
  • Species
    H. sapiens (human)
  • Mutation
    contains -82/+116 of promoter
  • Entrez Gene
    MAGEA1 (a.k.a. CT1.1, MAGE1)
  • Promoter MAGE-A1
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAGE-A1Pro pGL4basic was a gift from Heidi Schwarzenbach (Addgene plasmid # 78136 ; http://n2t.net/addgene:78136 ; RRID:Addgene_78136)
  • For your References section:

    Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters. Wischnewski F, Friese O, Pantel K, Schwarzenbach H. Mol Cancer Res. 2007 Jul;5(7):749-59. 10.1158/1541-7786.MCR-06-0364 PubMed 17634428