MXS_BidirectionalCAG
(Plasmid #78175)

• Purpose: Bidirectional CAG promoter (compatible with the MXS Chaining Kit)
• Depositing Lab: Pierre Neveu
• Publication: Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016.

Backbone

• Vector backbone: modified MXS_chaining vector Addgene 62394
• Backbone size w/o insert (bp): 1930
• Total vector size (bp): 6230
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bidirectional CAG Promoter
• Species: Synthetic
• Insert Size (bp): 4280

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TTACCGCCTTTGAGTGAG
• 3′ sequencing primer: TTGTCTCATGAGCGGATAC

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Due to the repeated CAG promoter sequences, a SpeI + EcoRI digest is recommended to confirm the integrity of the chicken-actin/beta globin promoter fragments. Please see link to a diagnostic digest image in the Resource Information section above.