pDHJS3 AN-
(Plasmid #78241)

• Purpose: One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).
• Depositing Lab: Tao-shih Hsieh
• Publication: Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6. Epub 2006 Apr 11.

Backbone

• Vector backbone: pBluescript SK-
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2958
• Total vector size (bp): 3413
• Modifications to backbone: Inserted two loxP sites with sequence "A" (from Drosophila Top3a) in between

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: This plasmid requires a helper phagemid to make single stranded DNA. Suggested helper phaemids: M13K07 (available from NEB) or pM13g (available from Andrew Bradbury at Los Alamos National Laboratory- PMID: 17088290). If using M13K07 helper phagemid, transform plasmid DNA in XL2 MRF'. If using pM13g helper phagemid, transform plasmid DNA in DH5alpha.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: loxP
• Species: P1
• Insert Size (bp): 34

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: sequence "A" with NheI site
• Species: D. melanogaster (fly)
• Insert Size (bp): 300

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: none

Gene/Insert 3

• Gene/Insert name: loxP2
• Species: P1
• Insert Size (bp): 34

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NdeI (not destroyed)
• 5′ sequencing primer: none

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry