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pCIG3 (pCMV-IRES-GFP version 3)
(Plasmid #78264)


Item Catalog # Description Quantity Price (USD)
Plasmid 78264 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.


  • Vector backbone
    Derived from pCIG1
  • Backbone manufacturer
    Goodrum lab, Addgene plasmid 78265
  • Backbone size (bp) 7624
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter CMV, lentiviral LTR
  • Tag / Fusion Protein
    • IRES-EGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector differs from pCIG1 in the following manners:
- Different IRES (derived from Clontech pIRES-GFP), which is easier to mutagenize, PCR, and sanger sequence.
- Expanded MCS, derived from linker oligo cloning.
- No longer contains an unannotated second XbaI site. XbaI site in MCS can be used for cloning.

Information for Cloning Grade DNA (Catalog # ( Back to top )


Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.


  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG3 (pCMV-IRES-GFP version 3) was a gift from Felicia Goodrum (Addgene plasmid # 78264 ; ; RRID:Addgene_78264)
  • For your References section:

    Complex expression of the UL136 gene of human cytomegalovirus results in multiple protein isoforms with unique roles in replication. Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F. J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 2014 Oct 8. 10.1128/JVI.02711-14 PubMed 25297993