Cytosolic FAK biosensor
PurposeVisualization of cytosolic FAK activity in live cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78300||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCytosolic FAK biosensor
Alt nameECFP-SH2 domain of c-Src-substrate-YPet
Insert Size (bp)1800
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
We designed a FRET-based FAK biosensor, with a central piece containing the SH2 domain derived from c-Src, a flexible linker peptide, and a specific substrate sequence from FAK encompassing Tyr397, concatenated between ECFP and YPet.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Cytosolic FAK biosensor was a gift from Yingxiao Wang (Addgene plasmid # 78300 ; http://n2t.net/addgene:78300 ; RRID:Addgene_78300)
For your References section:Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Seong J, Ouyang M, Kim T, Sun J, Wen PC, Lu S, Zhuo Y, Llewellyn NM, Schlaepfer DD, Guan JL, Chien S, Wang Y. Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414. 10.1038/ncomms1414 PubMed 21792185