Cytosolic FAK biosensor
(Plasmid #78300)

• Purpose: FRET biosensor. Visualization of cytosolic FAK activity in live cells
• Depositing Lab: Yingxiao Wang
• Publication: Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cytosolic FAK biosensor
• Alt name: ECFP-SH2 domain of c-Src-substrate-YPet
• Species: Synthetic
• Insert Size (bp): 1800
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We designed a FRET-based FAK biosensor, with a central piece containing the SH2 domain derived from c-Src, a flexible linker peptide, and a specific substrate sequence from FAK encompassing Tyr397, concatenated between ECFP and YPet.