pTiGc
(Plasmid #78314)

• Purpose: Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
• Depositing Lab: Samantha Sampson
• Publication: Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/mic.0.000288.

Backbone

• Vector backbone: pST5552
• Backbone manufacturer: Seeliger et al., 2012 PLoS One. 7(1):e29266. Epub 2012 Jan 18
• Modifications to backbone: It contains hsp60 - gfp, hsp60(ribo) - turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch)
• Vector type: Bacterial Expression, Synthetic Biology ; Mycobacterial replicating vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TurboFP635
• Species: Synthetic
• Promoter: hsp60(ribo)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: GFP
• Species: Aequorea victoria
• Promoter: hsp60

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry