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HisMBP CFlm25
(Plasmid #78458)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78458 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST-HisMBP fusion vector
  • Backbone manufacturer
    David Waugh, National Cancer Institute, NIH
  • Backbone size w/o insert (bp) 8282
  • Total vector size (bp) 8963
  • Vector type
    Bacterial Expression
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human Cleavage Factor lm 25 kDa subunit
  • Alt name
    CPSF5
  • Alt name
    NUDT21
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    681
  • GenBank ID
    11051
  • Entrez Gene
    NUDT21 (a.k.a. CFIM25, CPSF5)
  • Promoter tac
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • MBP (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original CFlm25 gene came from Greg Gilmartin (University of Vermont).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Has a TEV cleavage site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisMBP CFlm25 was a gift from Sylvie Doublie (Addgene plasmid # 78458 ; http://n2t.net/addgene:78458 ; RRID:Addgene_78458)
  • For your References section:

    Crystal structure of the 25 kDa subunit of human cleavage factor Im. Coseno M, Martin G, Berger C, Gilmartin G, Keller W, Doublie S. Nucleic Acids Res. 2008 Jun;36(10):3474-83. doi: 10.1093/nar/gkn079. Epub 2008 Apr 29. 10.1093/nar/gkn079 PubMed 18445629