pCM18
(Plasmid
#78565)
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Purpose(Empty Backbone) 60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSEC84
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- luxCDABE (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- luxCDABE (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM18 was a gift from James Kaper (Addgene plasmid # 78565 ; http://n2t.net/addgene:78565 ; RRID:Addgene_78565) -
For your References section:
Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine strain CVD 103-HgR. Morin CE, Kaper JB. FEMS Immunol Med Microbiol. 2009 Oct;57(1):69-79. doi: 10.1111/j.1574-695X.2009.00580.x. Epub 2009 Jul 1. 10.1111/j.1574-695X.2009.00580.x PubMed 19678844
