Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pWalium20-10XUAS-3XFLAG-dCas9-VPR Citations (9)

Originally described in: In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila.
Lin S, Ewen-Campen B, Ni X, Housden BE, Perrimon N Genetics. 2015 Oct;201(2):433-42. doi: 10.1534/genetics.115.181065. Epub 2015 Aug 5.
PubMed Journal

Articles Citing pWalium20-10XUAS-3XFLAG-dCas9-VPR

Articles
Optimized strategy for in vivo Cas9-activation in Drosophila. Ewen-Campen B, Yang-Zhou D, Fernandes VR, Gonzalez DP, Liu LP, Tao R, Ren X, Sun J, Hu Y, Zirin J, Mohr SE, Ni JQ, Perrimon N. Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9409-9414. doi: 10.1073/pnas.1707635114. Epub 2017 Aug 14. PubMed
Synthetically engineered Medea gene drive system in the worldwide crop pest Drosophila suzukii. Buchman A, Marshall JM, Ostrovski D, Yang T, Akbari OS. Proc Natl Acad Sci U S A. 2018 Apr 17. pii: 1713139115. doi: 10.1073/pnas.1713139115. PubMed

Associated Plasmids

A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues. Huynh N, Zeng J, Liu W, King-Jones K. G3 (Bethesda). 2018 Nov 6;8(11):3593-3605. doi: 10.1534/g3.118.200539. PubMed
Spatial and temporal control of gene manipulation in Drosophila via drug-activated Cas9 nucleases. Huynh N, Wang S, King-Jones K. Insect Biochem Mol Biol. 2020 May;120:103336. doi: 10.1016/j.ibmb.2020.103336. Epub 2020 Feb 24. PubMed
A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning. Schuller A, Wolansky L, Berger H, Studt L, Gacek-Matthews A, Sulyok M, Strauss J. Appl Microbiol Biotechnol. 2020 Nov;104(22):9801-9822. doi: 10.1007/s00253-020-10900-9. Epub 2020 Oct 2. PubMed
Development of a miRNA Sensor by an Inducible CRISPR-Cas9 Construct in Ciona Embryogenesis. Wang Z, Sun X, Zhang X, Dong B, Yu H. Mol Biotechnol. 2021 Jul;63(7):613-620. doi: 10.1007/s12033-021-00324-9. Epub 2021 Apr 20. PubMed
Engineered reproductively isolated species drive reversible population replacement. Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, Perry MW, Akbari OS. Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z. PubMed

Associated Plasmids

Parasitic nematode fatty acid- and retinol-binding proteins compromise host immunity by interfering with host lipid signaling pathways. Parks SC, Nguyen S, Nasrolahi S, Bhat C, Juncaj D, Lu D, Ramaswamy R, Dhillon H, Fujiwara H, Buchman A, Akbari OS, Yamanaka N, Boulanger MJ, Dillman AR. PLoS Pathog. 2021 Oct 29;17(10):e1010027. doi: 10.1371/journal.ppat.1010027. eCollection 2021 Oct. PubMed

Associated Plasmids

Base Editing of Somatic Cells Using CRISPR-Cas9 in Drosophila. Marr E, Potter CJ. CRISPR J. 2021 Dec;4(6):836-845. doi: 10.1089/crispr.2021.0062. Epub 2021 Nov 23. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.