SaMscL (CDelta26)
(Plasmid
#79027)
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PurposeStaphylococcus aureus MscL with C Terminal Truncation of 26 amino acids
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5680
- Total vector size (bp) 6063
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Modifications to backboneNone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMscL
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Alt nameSaMscL (CDelta26)
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SpeciesStaphylococcus aureus
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Insert Size (bp)363
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MutationStop codon placed before 26 amino acids at C Terminal of MscL
- Promoter T7 Promoter
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Tag
/ Fusion Protein
- 6x Histidine Tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SaMscL (CDelta26) was a gift from Douglas Rees (Addgene plasmid # 79027 ; http://n2t.net/addgene:79027 ; RRID:Addgene_79027) -
For your References section:
Structure of a tetrameric MscL in an expanded intermediate state. Liu Z, Gandhi CS, Rees DC. Nature. 2009 Sep 3;461(7260):120-4. doi: 10.1038/nature08277. Epub 2009 Aug 23. 10.1038/nature08277 PubMed 19701184
