GFP-KIF1B beta-FL
(Plasmid #79108)

• Purpose: Mammalian expression of GFP-KIF1B beta-FL
• Depositing Lab: Susanne Schlisio
• Publication: Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi: 10.1158/2159-8290.CD-13-0362. Epub 2014 Jan 27.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-KIF1B beta full length
• Alt name: kinesin family member 1B
• Species: H. sapiens (human)
• Insert Size (bp): 5780
• Mutation: exon 25 missing (natural splice varient)
• Entrez Gene: KIF1B (a.k.a. CMT2, CMT2A, CMT2A1, HMSNII, KLP, NBLST1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry