pC003 - LshC2C2 locus into pACYC184 for spacer cloning
(Plasmid #79152)

• Purpose: LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning
• Depositing Lab: Feng Zhang
• Publication: Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Backbone

• Vector backbone: pACYC184
• Backbone size w/o insert (bp): 4723
• Total vector size (bp): 10444

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): STBL3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LshC2c2 locus
• Species: Leptotrichia shahii
• Mutation: *

Resource Information

• Supplemental Documents:
  • pC003 genbank file
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*There is a premature stop int he Cas2 protein, removing the final 22 aa's (MKVLAGEVTMGEKISQNKILEF). From the author: "The Cas1/Cas2 are not necessary for the purposes of the plasmid as we have shown that C2c2 is all that is necessary for RNA targeting. [Investigators] should use pC001 if they’re actually interested in the Cas proteins."