pETDuet-T7-Ct-YhjH
(Plasmid #79167)

• Purpose: Dual expression of Ct biosensor and YhjH under individual T7 promoters
• Depositing Lab: Ming Hammond
• Publication: Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw580.

Backbone

• Vector backbone: pETDuet
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5420
• Total vector size (bp): 6554
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: yhjH
• Species: E. coli
• Insert Size (bp): 769
• GenBank ID: EC:3.1.4.52
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gatcgagatcgatctcgatccc
• 3′ sequencing primer: ttgtacacggccgcataatc

Gene/Insert 2

• Gene/Insert name: T7-Spinach2-Ct
• Insert Size (bp): 236
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTGTACACGGCCGCATAATC
• 3′ sequencing primer: CGCTTAATGCGCCGCTACAG

Resource Information

• Supplemental Documents:
  • Also see https://benchling.com/s/rX15sMEA/edit
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry