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pSBDEST.IP
(Plasmid #79472)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79472 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSBDEST.IP
  • Backbone manufacturer
    cloned in house
  • Backbone size (bp) 7294
  • Vector type
    Promoterless Gateway Destination vector
  • Promoter none
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning. Uses an internal ribosomal entry site to tie Puromycin selection to expression of inserted transgene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBDEST.IP was a gift from Peter Jon Nelson (Addgene plasmid # 79472 ; http://n2t.net/addgene:79472 ; RRID:Addgene_79472)
  • For your References section:

    A vector platform for the rapid and efficient engineering of stable complex transgenes. Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner E, Nelson PJ. Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365. 10.1038/srep34365 PubMed 27694838