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pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
(Plasmid #79619)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79619 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 10162
  • Modifications to backbone
    Neomycin marker is connected to insert via 2A peptide
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Species
    Streptococcus pyogenes
  • Mutation
    D10A for nickase Cas9
  • Promoter pCMV
  • Tag / Fusion Protein
    • 3xFlag-PmCDA1 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttcgctattacgccagctg
  • 3′ sequencing primer GACAGGTCGGTCTTGACA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT) was a gift from Akihiko Kondo (Addgene plasmid # 79619 ; http://n2t.net/addgene:79619 ; RRID:Addgene_79619)
  • For your References section:

    Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. Science. 2016 Aug 4. pii: aaf8729. 10.1126/science.aaf8729 PubMed 27492474