Purpose(Empty Backbone) Staging plasmid for use in DIP transposition
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79770||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2318
Modifications to backboneBsaI and BsmBI sites removed
Vector typeSynthetic Biology ; Staging plasmid for transposition reactions
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Clone ORF into the vector with BsaI. Clone ORF out of vector with BsmBI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pATT-Dest was a gift from David Savage (Addgene plasmid # 79770 ; http://n2t.net/addgene:79770 ; RRID:Addgene_79770)
For your References section:Rapid construction of metabolite biosensors using domain-insertion profiling. Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF. Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266. 10.1038/ncomms12266 PubMed 27470466