pNPK-NLS-Vika
(Plasmid #79969)

• Purpose: expresses human codon-optimized Vika-recombinase in mammalian cells
• Depositing Lab: Frank Buchholz
• Publication: Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. doi: 10.1093/nar/gks1037. Epub 2012 Nov 9.

Backbone

• Vector backbone: pNPK
• Total vector size (bp): 6005
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human codon-optimized Vika sequence, NLS tagged
• Species: Synthetic; derived from Vibrio coralliilyticus ATCC BAA-450
• Insert Size (bp): 1110
• GenBank ID: NZ_ACZN01000014.1 ZP_05884863
• Promoter: PGK
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GCTGTTCTCCTCTTCCTC
• 3′ sequencing primer: CTCTGTAGGTAGTTTGTCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry