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EGFP gRNA (BRDN0000561167)
(Plasmid #80034)


Item Catalog # Description Quantity Price (USD)
Plasmid 80034 Standard format: Plasmid sent in bacteria as agar stab 1 $65
Lentiviral Prep 80034-LV

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Zhang Lab (Addgene plasmid # 52963)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • gRNA/shRNA sequence
  • Species
  • Promoter hU6

Cloning Information

Resource Information

Depositor Comments

Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at or visit for detailed protocols and information.

Information for Lentiviral Prep (Catalog # 80034-LV) ( Back to top )


Ready-to-use Lentiviral Prep particles produced from EGFP gRNA (BRDN0000561167) (#80034). In addition to the viral particles, you will also receive purified EGFP gRNA (BRDN0000561167) plasmid DNA.

Lentiviral particles carrying an EGFP sgRNA (BRDN0000561167) and puromycin resistance.


  • Volume 1mL
  • Titer ≥1x10⁶ TU/mL
  • Pricing $100 USD for preparation of 1mL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids psPAX2 (plasmid #12260)
  • Envelope pMD2.G (plasmid #12259)
  • Buffer DMEM +10% FBS
  • Selectable Marker Puromycin


Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Resource Information

Viral Quality Control

Titering Method:
  • Colony formation assay: A549 cells were transduced with serial dilutions of 80034-LV and treated with puromycin. Puromycin-resistant colonies were expanded for approximately 2 weeks, stained with crystal violet, and counted.
  • PCR confirmation of insert: PCR was carried out with primers targeting the hU6 and EF1-alpha promoters. The PCR product was visualized on an agarose gel for size confirmation, purified and the guide sequence was confirmed by Sanger sequencing.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP gRNA (BRDN0000561167) was a gift from John Doench & David Root (Addgene plasmid # 80034 ; ; RRID:Addgene_80034)

    For viral preps, please replace (Addgene plasmid # 80034) in the above sentence with: (Addgene viral prep # 80034-LV)

  • For your References section:

    Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. 10.1038/nbt.3437 PubMed 26780180