PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80066||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6306
- Total vector size (bp) 8365
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
SpeciesD. rerio (zebrafish)
Insert Size (bp)2059
- Promoter Beta-actin2
/ Fusion Protein
- 3x HA (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer atttaggtgacactatagaagag
- 3′ sequencing primer taatacgactcactatagggag (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMTB2-cytoBirA-2A-mCherry_Ras was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 80066)
For your References section:Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome. Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, Senanayake U, Fraser SE, Sauka-Spengler T. Cell Rep. 2017 Apr 11;19(2):425-440. doi: 10.1016/j.celrep.2017.03.045. 10.1016/j.celrep.2017.03.045 PubMed 28402863
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.