PU.1-ets-IRES-mCherry
(Plasmid
#80141)
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Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneIRES-mCherry
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Backbone manufacturerRothernberg lab, Addgene plasmid 80139
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePU.1
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SpeciesM. musculus (mouse)
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Mutationets = PU.1 DNA binding domain (amino acids 159-262)
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Entrez GeneSpi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
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Tag
/ Fusion Protein
- IRES-mCherry (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PU.1-ets-IRES-mCherry was a gift from Ellen Rothenberg (Addgene plasmid # 80141 ; http://n2t.net/addgene:80141 ; RRID:Addgene_80141) -
For your References section:
Positive feedback between PU.1 and the cell cycle controls myeloid differentiation. Kueh HY, Champhekar A, Nutt SL, Elowitz MB, Rothenberg EV. Science. 2013 Aug 9;341(6146):670-3. doi: 10.1126/science.1240831. Epub 2013 Jul 18. 10.1126/science.1240831 PubMed 23868921
