RP4-6
(Plasmid #80385)

• Purpose: Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant
• Depositing Lab: Michael Hynes
• Publication: Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Backbone

• Vector backbone: RP4
• Backbone manufacturer: Natural
• Backbone size w/o insert (bp): 60000
• Total vector size (bp): 60000
• Modifications to backbone: Spc gene added inserted into Tc gene by in vivo cloning. See article.
• Vector type: Helper BHR plasmid for mobilizing other plasmids carrying oriT

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): CSH56
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Spectinomycin/Streptomycin
• Alt name: Spc
• Species: from Omega interposon
• Insert Size (bp): 2100
• Promoter: None
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was made by in vivo cloning as described in the article. The Spc/Sm cassette was cloned into a suicide vector carrying the RP4 Tc resistance gene, and this was recombined into RP4-4, a Km sensitive derivative of RP4.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Provides a differently marked (Spc) helper plasmid for mobilization of plasmids and chromosomes carrying an RP4 or RK2 mob region (oriT), such as plasmids or chromosomes tagged with a Tn5-Mob derivative.

Depositor states that growth on respective antibiotic is sufficient to confirm plasmid integrity.