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NLS-AgDD
(Plasmid #80625)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80625 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PiggyBac transformation vector pB
  • Backbone size w/o insert (bp) 6652
  • Total vector size (bp) 7763
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-NES-L106P-sfGFP
  • Alt name
    NLS-AgDD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1113
  • GenBank ID
    NM_000801 AB971579
  • Tag / Fusion Protein
    • MPPKKKRKVLALKLAGLDI (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer YM213--5' atgccttcttctttttcctacagct
  • 3′ sequencing primer YM215--5' gatgagtttggacaaaccacaact
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Depositor reference sequence and Addgene Sanger results show F36V compared to NM_000801.4.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-AgDD was a gift from Thomas Wandless (Addgene plasmid # 80625)
  • For your References section:

    A method to rapidly create protein aggregates in living cells. Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, Wandless TJ. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689. 10.1038/ncomms11689 PubMed 27229621