BAP1
(Plasmid #81024)

• Purpose: Retroviral vector containing human BAP1 and Thy1.1 selection marker
• Depositing Lab: Anjana Rao
• Publication: Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.

Backbone

• Vector backbone: MSCV-IRES-Thy1.1 DEST
• Backbone manufacturer: Rao lab (Addgene plasmid# 17442)
• Backbone size w/o insert (bp): 8200
• Total vector size (bp): 10400
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Thy1.1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAP1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
• Promoter: 5’LTR

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pLXSN-5 (5'-CCCTTGAACCTCCTCGTTCGACC-3')
• 3′ sequencing primer: IRES-R (5'-GCATTCCTTTGGCGAGAG-3')

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry