This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pDONR223_PIK3CA_p.H1047Y
(Plasmid #81701)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 81701 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2790
  • Vector type
    Gateway Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIK3CA
  • Alt name
    NM_006218.3
  • Species
    H. sapiens (human)
  • Mutation
    H1047Y
  • Entrez Gene
    PIK3CA (a.k.a. CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
  • Promoter None

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Closed state: stop codon immediately follows insert

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223_PIK3CA_p.H1047Y was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 81701)
  • For your References section:

    Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. 10.1158/2159-8290.CD-16-0160 PubMed 27147599