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pVHed07
(Plasmid #82350)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82350 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    R6K
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 4990
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir2+
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mKate2 RFP
  • Insert Size (bp)
    696

Gene/Insert 2

  • Gene/Insert name
    Superfolder GFP
  • Insert Size (bp)
    720

Gene/Insert 3

  • Gene/Insert name
    Temporal logic gate with TP901 and Bxb1 attachment sites
  • Insert Size (bp)
    394

Resource Information

Depositor Comments

This plasmid has a R6K ori requires a Pir2+ strain to replicate. When this plasmid is co-transformed with pAH123 (Addgene #66077) and grown at 37, the plasmid is linearized and integrated into the Phi80 site on the E. coli chromosome. The use of the R6K origin ensures that non-integrated plasmids will not replicate in the target strain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVHed07 was a gift from Richard Murray (Addgene plasmid # 82350 ; http://n2t.net/addgene:82350 ; RRID:Addgene_82350)
  • For your References section:

    A population-based temporal logic gate for timing and recording chemical events. Hsiao V, Hori Y, Rothemund PW, Murray RM. Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663. PubMed 27193783