PurposesgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||82386||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6423
Growth in Bacteria
Copy numberHigh Copy
- Promoter PEZ3
- Cloning method Gibson Cloning
- 5′ sequencing primer TTGGGTAACGCCAGGGT
- 3′ sequencing primer GCTTCCGGCTCGTATGTTGTG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCas34 was a gift from Brian Pfleger (Addgene plasmid # 82386 ; http://n2t.net/addgene:82386 ; RRID:Addgene_82386)
For your References section:CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002. Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger BF. Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 10.1016/j.ymben.2016.07.007. 10.1016/j.ymben.2016.07.007 PubMed 27481676