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pPlatTET-gRNA2
(Plasmid #82559)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82559 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha ; TOP10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCAG vector is from Dr. Dr Jun-ichi Miyazaki. The dCas9 fragment was amplified from Addgene plasmid 60903 by PCR. The GFP fragment was amplified from Addgene plasmid 60904. The scFv fragment was also amplified from Addgene plasmid 60904.
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPlatTET-gRNA2 was a gift from Izuho Hatada (Addgene plasmid # 82559 ; http://n2t.net/addgene:82559 ; RRID:Addgene_82559)
  • For your References section:

    Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I. Nat Biotechnol. 2016 Aug 29. doi: 10.1038/nbt.3658. 10.1038/nbt.3658 PubMed 27571369