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p206_LTJ_sgRNACD45.2_R3
(Plasmid #82674)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82674 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pX458
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid# 48138)
  • Backbone size w/o insert (bp) 9300
  • Total vector size (bp) 9300
  • Modifications to backbone
    sgRNA cloned into the BbsI site of pX458
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting CD45.2 region 3
  • gRNA/shRNA sequence
    GTAGGTCCGGACAAGGTCAA
  • Species
    M. musculus (mouse)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer U6-forward primer (ACTATCATATGCTTACCGTAAC)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p206_LTJ_sgRNACD45.2_R3 was a gift from Lukas Jeker (Addgene plasmid # 82674 ; http://n2t.net/addgene:82674 ; RRID:Addgene_82674)
  • For your References section:

    Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells. Kornete M, Marone R, Jeker LT. J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121. 10.4049/jimmunol.1701121 PubMed 29445007