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pET_sfCherry(1-10)_32aalinker_sfCherry(11)
(Plasmid #83030)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83030 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5296
  • Total vector size (bp) 6067
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Transform plasmid into BL21 for protein expression. Induce protein expression by IPTG.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfCherry(1-10)_32aalinker_sfCherry(11)
  • Alt name
    split sfCherry flanked by 32-residue linker
  • Species
    Synthetic
  • Insert Size (bp)
    771
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_sfCherry(1-10)_32aalinker_sfCherry(11) was a gift from Bo Huang (Addgene plasmid # 83030 ; http://n2t.net/addgene:83030 ; RRID:Addgene_83030)
  • For your References section:

    Improved split fluorescent proteins for endogenous protein labeling. Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B. Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8. 10.1038/s41467-017-00494-8 PubMed 28851864