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pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro
(Plasmid #83107)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83107 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti PGK Puro DEST (w529-2)
  • Backbone manufacturer
    Eric Campeau - Addgene plasmid #19068
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    c-Myc
  • Species
    H. sapiens (human)
  • Mutation
    p64 isoform
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter hPGK
  • Tag / Fusion Protein
    • 2A self cleaving peptide (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CDK4
  • Species
    H. sapiens (human)
  • Mutation
    R24C
  • Entrez Gene
    CDK4 (a.k.a. CMM3, PSK-J3)
  • Promoter hPGK (single transcript)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    eGFP
  • Species
    jellyfish
  • Promoter hPGK (single transcript)

Cloning Information for Gene/Insert 3

Resource Information

Depositor Comments

This construct contains the p64 isoform of c-Myc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro was a gift from Andrew Brooks (Addgene plasmid # 83107 ; http://n2t.net/addgene:83107 ; RRID:Addgene_83107)