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pmNFM
(Plasmid #83126)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83126 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1 with EGFP sequence removed
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4047
  • Total vector size (bp) 6594
  • Modifications to backbone
    EGFP sequence removed using Age I and BspE I
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse NFM
  • Alt name
    mNFM
  • Alt name
    neurofilament protein M
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2547
  • GenBank ID
    DQ201636
  • Entrez Gene
    Nefm (a.k.a. NF-M, NF160, NF165, Nef3, Nfm)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.

Mouse NFM cDNA sequence deposited in Genbank (Accession number DQ201636).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmNFM was a gift from Anthony Brown (Addgene plasmid # 83126)
  • For your References section:

    The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons. Yan Y, Jensen K, Brown A. Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309. 10.1002/cm.20184 PubMed 17285620